Simultaneous Determination of Gallic Acid and Berberine Hydrochloride in Dihuang Sprays by HPLC
- VernacularTitle:HPLC法同时测定地黄喷雾剂中没食子酸和盐酸小糪碱含量
- Author:
Yan LI
;
Ligang ZHAO
;
Chunyan WANG
;
Guilan YU
;
Qi YANG
;
Hongmin WANG
- Publication Type:Journal Article
- Keywords:
Dihuangsprays;
Gallicacid;
Berberinehydrochloride;
HPLC;
Contentdetermination
- From:
China Pharmacist
2014;(10):1682-1683,1686
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish an HPLC method for the determination of gallic acid and berberine hydrochloride in Dihuang sprays. Methods:A ZORBAX SB-C18 column (250 mm × 4. 6 mm,5 μm) was used, the mobile phase was acetonitrile-0. 1% phos-phoric acid with gradient elution. The flow rate was 1. 0 ml·min-1 , the column temperature was 30℃, and the detection wavelength wassetat270nm.Results:Thelinearrangeofgallicacidandberberinehydrochloridewas20.36-305.40 μg·ml-1(r=0.9996) and 20.08-301.20 μg·ml-1(r=0.999 8), respectively. The average recovery was 98.9%(RSD =1.3%) and 98.5%(RSD =1. 5%) , respectively. Conclusion:The method is simple, reliable and accurate, which can be used in the determination of gallic acid and berberine hydrochloride of Dihuang sprays.
