Determination of Two Ingredients in Losartan Potassium and Hydrochlorothiazide Tablets by HPLC
- VernacularTitle:HPLC法测定氯沙坦钾氢氯噻嗪片中两组分的含量
- Author:
Tingting LI
- Publication Type:Journal Article
- Keywords:
Losartanpotassium;
Hydrochlorothiazide;
Determination;
HPLC
- From:
China Pharmacist
2014;(10):1684-1686,1683
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish an HPLC method for the determination of two ingredients in losartan potassium and hydrochlo-rothiazide tablets. Methods: Losartan potassium and hydrochlorothiazide were separated on a Zorbax Eclipse XDB-C8 ( 200 mm × 4. 6 mm, 5 μm)column, the mobile phase consisted of 0. 01 mol·L-1 monopotassium phosphate solution(adjusting pH to 3. 0 with phosphoric acid)-acetonitrile(70∶30)with the flow rate of 1. 0 ml·min-1. The column temperature was set at 35℃, the detection wavelength was 271nm and the injection volume size was 20 μl. Results:The linear range of losartan potassium and hydrochlorothiaz-ide was 39.63-118.89 μg·ml-1(r=0.999 9) and 9.47-28.41 μg·ml-1(r=0.999 6) with the average recovery of 99.4%(RSD=1. 0%,n=9)and 99. 8%(RSD=0. 9%, n=9), respectively. Conclusion: The method is simple, fast, accurate and reproduci-ble, which is suitable for the determination of losartan potassium and hydrochlorothiazide tablets.
