Analysis of RAC1 gene polymorphism in Hubei Chinese healthy population
10.3969/j.issn.1000-484X.2014.10.001
- VernacularTitle:中国湖北地区健康人群RAC1基因多态性的测定与分析
- Author:
Jiali ZHOU
;
Yani LIU
;
Rufei SHEN
;
Chunxiao YANG
;
Xiaomei LUO
;
Yu ZHANG
;
Shaojun SHI
- Publication Type:Journal Article
- Keywords:
Rac1;
Single nucleotide polymorphisms;
Genetic sequencing;
Healthy Hubei Han population
- From:
Chinese Journal of Immunology
2014;(10):1297-1301,1308
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a method for the detection of 8 single nucleotide polymorphisms (SNPs) of RAC1 gene,and to analyze the genotypic and allelic distributions of the 8 SNPs in healthy Chinese Han population.Methods:The real-time fluorescence TaqMan-MGB probes allele classification technology was used for the determination of the 8 SNPs in 150 cases of healthy volunteers in Hubei China ,and the results were validated by direct gene sequencing.Results: The method established in this study can accurately screen the genotypes of the 8 SNPs of human RAC1 gene.Highly linkage disequilibriums were found between rs 10951982 and rs9374 , rs702482 and 836488 ,respectively.All the 8 sits were in accordance with Hardy-Weinberg equilibrium.Six Tag-SNPs were selected by Heploview software:rs10951982 ,rs6954996 ,rs6951997 ,r1s2977 ,rs702482 and rs702483.The MAFs of the 8 SNPs in our study were close to the MAFs in CHB and Asian in Hap Map database .Significant distribution differences were also observed in different races.Conclusion:No significant differences are observed in this study in healthy Chinese Han population.But differences are found when compared with the data of other races in Hap Map.
