Effect of Slug knock-down on tumor formation and metastasis of HCT116 cells in nude mice
10.3969/j.issn.1000-484X.2014.10.003
- VernacularTitle:RNA干扰Slug基因对HCT116细胞裸鼠移植瘤生长及转移的影响
- Author:
Jiang QIAN
;
Jia HAN
;
Peng CHEN
;
Chunli ZHU
;
Zhongxue FU
- Publication Type:Journal Article
- Keywords:
RNA interference;
Slug;
Nude mice;
HCT116
- From:
Chinese Journal of Immunology
2014;(10):1309-1312,1319
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the influence of RNAi mediated Slug silencing on the growth and metastasis of colon cancer in nude mice.Methods: HCT116 colon cancer cells use for 24 five-week-old nude mice implanted subcutaneously , established colon cancer xenograft model in nude mice ,all divided into blank control group ,negative control group and the experimental group ,each group had eight nude mices.All group were injected with saline , negative plasmid and lentivirus vectors respectively.Tumor growth was observed and draw tumor curved growth ,changes in tumor growth and lymph node metastasis between the groups were observed ,Slug gene and protein expression were detected by immunohistochemistry ,qRT-PCR and Western blot analysis.Results: Slug gene shRNA intervention group compared with the control group and negative control group ,tumor grew slower ,tumor mass was significantly reduced (3.08±0.31 vs 7.37±1.18,7.46±1.16,P＜0.01),experimental group of lymph node-positive rate was 36.3%( 4 /11 ) ,compared to the negative control group 77.8% ( 14/18 ) and the control group was 68.4% ( 13/19 ) ( P＜0.01 ).Conclusion: Targeted Slug RNA interference can significantly inhibit the growth of colon cancer in nude mice ,lymph node metastasis and the expression of the gene protein in cancer tissue ,Slug may be a potential molecular target for colon cancer gene therapy.
