Comparison of Brucellacapt and iELISA in serological diagnosis of brucellosis

VernacularTitle:布鲁氏菌病血清学Brucellacapt和iELISA检测方法的比较
Author: Na ZHAO ; Chihong ZHAO ; Rong RONG ; Pei XIAO ; Yan SUN ; Na WANG ; Buyun CUI
Publication Type:Journal Article
Keywords: brucellosis; diagnosis; Brucellacapt; iELISA
From: Chinese Journal of Zoonoses 2014;(10):1045-1047,1051
CountryChina
Language:Chinese
Abstract: Brucellacapt is a new serological test based on immunocapture-agglutination technique that has been used in many countries ,but there was no related article reported in China .The value of the Brucellacapt method in diagnosing human brucellosis was evaluated in this study .Among 120 suspected patients ,75 patients and 45 negatives were diagnosed by SAT and RBPT method combination with their clinical symptoms .Sera from all 120 people were tested by the method of Brucella-capt and iELISA ,and the results were ,consequently ,analyzed and compared .It showed that sensitivity ,specificity ,consis-tency rate ,Kappa value ,and area under ROC curve were found to be 82 .7% ,88 .9% ,85 .0% ,0 .69 ,and 0 .86 ,respectively for Brucellacapt ,whereas they were found to be 90 .7% ,64 .4% ,80 .8% ,0 .57 ,and 0 .78 for iELISA .In conclusion ,specific-ity ,consistency rate ,Kappa value ,and area under ROC curve were higher in Brucellacapt method than that in iELISA .How-ever ,the sensitivity of iELISA is higher .