Sequential immune’s effects of traditional BCG primary immunization and IL-12 combined with Ag85A DNA booster immunization in mice
10.3969/cjz.j.issn.1002-2694.2014.10.003
- VernacularTitle:传统 BCG 初免 IL-12联合 Ag85A DNA 疫苗加强序贯免疫小鼠效果观察
- Author:
Chan WANG
;
Xinmin WANG
;
Rong JI
;
Yuqing ZHANG
;
Feiyu WANG
;
Jiangdong WU
;
Fang WU
;
Wanjiang ZHANG
;
Le ZHANG
- Publication Type:Journal Article
- Keywords:
Mycobacterium tuberculosis;
sequential immunization strategy;
antigen 85A;
BCG;
interleukin
- From:
Chinese Journal of Zoonoses
2014;(10):997-1001,1008
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this study is to investigate the immune effects of BCG primary immunization and IL-12 combined with Mycobacterium tuberculosis Ag85A DNA vaccine booster immunization on mice .We randomly divided the mice into 7 groups ,namely PBS negative controls ,BCG controls ,pcAg85A controls ,BCG primary immunization combined with Ag85A booster immunization controls ,BCG primary immunization combined with Ag85A and IL-12 booster immunization controls , BCG primary immunization combined with IL-12 booster immunization controls ,and BCG primary immunization combined with pcDNA3 .1 booster immunization controls .Implementing the immune in procedure of BCG primary immunization and cytokine IL-12 booster immunization combined with mycobacterium tuberculosis Ag85A DNA ,we observed the immune effect on mice by detecting the mice serum total IgG , specific lymphocyte proliferation and cytokine levels in 4 ,6 ,8 weeks after the last immunization .Comparing the mice immunized in the strategy of BCG primary immunization and cytokine IL-12 combined with Mycobacterium tuberculosis Ag85A DNA vaccine strengthening immunization to the mice in other groups by other immune ways ,we found that ,in BCG/Ag85A+ IL-12 groups ,IgG in-creased significantly (P＜ 0 .05) ,specific lymphocyte prolifera-ted significantly ,and after strengthening immunization IFN-γlevels ,IL-2 levels and IL-4 levels in the three periods were 128 .2 ±20.4,190.2±16.51,244.2±39.14 ;146.2±17.29,271.6±16.36and16.36±28.12 ;68.6±6.62,96.6±5.5and5.5± 10 .71 ,respectively ,which were higher than those in other groups (P＜0 .05) .It’s suggested that the immunization way of BCG primary immunization and cytokine IL-12 combined with Mycobacterium tuberculosis Ag85A DNA vaccine booster immu-nization could significantly enhance the humoral and cellular immunity of the bodies ,and provide the basis for further study on protective effect test in animals .
