Homologous modeling and function analysis on thioredoxin glutathione reductase from Schistosoma j aponicum
10.3969/cjz.j.issn.1002-2694.2014.10.005
- VernacularTitle:日本血吸虫硫氧还蛋白谷胱甘肽还原酶同源建模及功能分析
- Author:
Jingwei HUANG
;
Yutong ZHENG
;
Jiahuang LI
;
Zichun HUA
- Publication Type:Journal Article
- Keywords:
thioredoxin glutathione reductase;
Schistosoma j aponicum;
homologous modeling;
function analysis
- From:
Chinese Journal of Zoonoses
2014;(10):1009-1013,1023
- CountryChina
- Language:Chinese
-
Abstract:
To explore the structure and function of thioredoxin glutathione reductase (TGR) from Schistosoma j aponi-cum ,the homologous model of TGR in Schistosoma j aponicum was constructed by Swiss-Pdbviewer based on sequence and structure alignment .The potential substrates binding sites of TGR were analyzed and these sites of various TGRs were also as-sessed .Our results showed that the homologous model of Schistosoma japonicum TGR based on Schistosoma mansoni TGR structure was proved to be reasonable by PROCHECK program .Analysis of binding sites showed that NADPH and GDS bind-ing sites were conservative sites and GSH binding site was a specific site for parasite .Our data suggested that inhibitors which work in NADPH and GDS binding sites of other various TGRs may also interact with TGR form Schistosoma j aponicum .GSH binding region might be one of the potential targets for design of specific inhibitors of parasite TGRs .In addition ,C-terminal of TGR plays an important role in electron transfer and may participate in the binding of the substrate .Thus compound inhibiting swing of C-terminal could effectively restrain Schistosoma j aponicum TGR activity .
