Suckling mouse model establishment of myocarditis induced by foot-and-mouth disease vi rus
10.3969/cjz.j.issn.1002-2694.2014.10.001
- VernacularTitle:口蹄疫病毒致乳鼠心肌炎的动物模型
- Author:
Keshan ZHANG
;
Jijun HE
;
Jiajun WU
;
Yongjie LIU
;
Weiwei CHENG
;
Youjun SHANG
;
Xiangtao LIU
;
Xuepeng CAI
- Publication Type:Journal Article
- Keywords:
suckling mice;
FMDV;
myocarditis;
animal model
- From:
Chinese Journal of Zoonoses
2014;(10):987-989,996
- CountryChina
- Language:Chinese
-
Abstract:
Interactions between FMDV and cardiac cells are multifaceted and complex ,these interactions leads to pro-teins alterations in cardiac cells inevitably .To understand the pathogenesis of myocarditis after FMDV infection in mice ,the suckling mouse model for myocarditis induced by foot-and-mouth disease virus (FMDV) was established in this study .Suckling mice within 3 days old was selected to infect by FMDV .Myocarditis caused by FMDV in suckling mice was confirmed with clinical symptom monitor .The observation of Hematoxylin and eosin stain (H&E stain) and transmission electron microscopy (TEM) were performed after samples processing .According to conventional polymerase chain reaction (PCR) methods ,prim-ers of VP1 gene was designed ,synthesised and specific FMDV VP1 gene was amplified from the heart muscle of suckling mice . The results indicated that suckling mice appeared low spirit condition ,dyspnea ,and dull reaction within 36 hours after chal-lenge with FMDV .Infiltration of inflammatory cells and dissolution of myocardial fibers were observed with H&E stain and TEM .Special target gene of FMDV was amplified from the heart of infected group .Obvious inflammation in the heart of suck-ling mice caused by FMDV was observed .It's suggested that suckling mouse model for myocarditis induced by FMDV was es-tablished successfully ,which would lay the foundation for researches of myocarditis mechanism in young cloven-hoofed ani-mals .
