Construction of TNF-αsiRNA Expression Vector and its Therapeutic Effect on TNF-αand IL-1 in TypeⅡCollagen Induced Arthritis Rat
10.3969/j.issn.0253-9896.2013.10.015
- VernacularTitle:TNF-αsiRNA表达载体的构建及其对胶原性关节炎大鼠TNF-α及IL-1的影响
- Author:
Chunyan PANG
;
Jianxin HUO
;
Fengfeng Lü
;
Zhifang CHANG
;
Yongfu WANG
- Publication Type:Journal Article
- Keywords:
tumor necrosis factor-alpha;
RNA;
small interfering;
interleukin-1;
arthritis;
experimental;
disease models;
animal
- From:
Tianjin Medical Journal
2013;(10):995-998,1045
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the therapeutic effect of tumor necrosis factor (TNF)-αsiRNA on typeⅡcolla-gen induced arthritis (CIA) in rats. Methods The expression vectors of siRNA against TNF-αgene were constructed suc-cessfully and were injected by tail veil into CIA rats. Twenty-four CIA rats were randomly divided into 4 groups including model group, empty vector group, TNF-α-siRNA1 group and TNF-α-siRNA2 group. CIA rats were injected with the same dose of phosphate buffered sodium (PBS) and pGFP-V-RS vector respectively in model group and empty vector group, while TNF-α-siRNA1 group and TNF-α-siRNA2 group were injected with TNF-α-siRNA1 eukaryotic expression vector and TNF-α-siRNA2 eukaryotic expression vector respectively. Another 6 rats, which were not established CIA model, were in-jected with PBS (blank control group). The serum expression levels of IL-1 were detected by ELISA on day 1, 5, 9 and 13 af-ter injection. The expression level of TNF-αmRNA was detected by reverse transcriptase polymerase chain reaction (RT-PCR) on day 13. Results The expression level of IL-1 was significantly higher on day 1, 5, 9 and 13 in model group than that of blank group (P＜0.05). The expression levels of IL-1 were significantly lower on day 1, 5 and 9 in TNF-α-siRNA1 group and TNF-α-siRNA2 group than that of model group and blank group (P ＜ 0.05). The expression level of TNF-αmRNA was significantly higher on day 13 in model group than that of blank group (P＜0.05). The expression levels of TNF-αmRNA were significantly lower in TNF-α-siRNA1 group and TNF-α-siRNA2 group than those of model group and emp-ty vector group (P＜0.05). Conclusion TNF-αspecific siRNA can suppress the levels of TNF-αmRNA and IL-1, which provides experimental basis for gene therapy of rheumatoid arthritis.
