Effects of Celecoxib on the Cell Migration, MMP-9 and VEGF Expression in Human Gastric Cancer SGC-7901
10.3969/j.issn.0253-9896.2013.10.005
- VernacularTitle:塞来昔布对人胃癌SGC-7901细胞迁移、MMP-9和VEGF表达的影响
- Author:
Yongxin WEI
;
Zhiguang ZHANG
;
Jiangming ZHONG
;
Man LI
- Publication Type:Journal Article
- Keywords:
stomach neoplasms;
prostaglandin-endoperoxide synthases;
matrix metalloproteinase 9;
vascular endo-thelial growth factors;
celecoxib
- From:
Tianjin Medical Journal
2013;(10):962-964,1042
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe selective cyclooxygenase-2 (COX-2) inhibitor celecoxib on the cell migration, MMP-9 and VEGF expression of human gastric cancer SGC-7901. Methods In vitro culture of SGC-7901 cells, the cells were divided into blank control group and celecoxib treatment group (different concentrations of celecoxib 25, 50 and 100μmol/L). SGC-7901 cells were treated with celecoxib for 24 h. The effect of celecoxib on cell migration of SGC-7901 was detected by the damage repair experiment. RT-PCR assay was used to detect the effect of celecoxib on MMP-9 mRNA and VEGF mRNA expressions in SGC-7901 cells. Results The migration distance of SGC-7901 cells was decreased with the increased concentration of celecoxib. There were significant differences between medium-dose and high-dose celecoxib groups and the control group. RT-PCR assay showed that MMP-9 mRNA and VEGF mRNA expressions in gastric cancer SGC-7901 were significantly decreased with the increased concentration of celecoxib, and there was a significant difference compared with those of control group (P＜0.05). Conclusion Celecoxib can inhibit migration of human gastric cancer SGC-7901 cells, which may be related to the inhibition of COX-2 downstream of MMP-9 and VEGF expressions.
