Silencing of MTA1 Attenuates Nasopharyngeal Carcinoma Metastasis in vitro
10.3969/j.issn.0253-9896.2014.04.006
- VernacularTitle:MTA1沉默抑制鼻咽癌转移能力的体外研究
- Author:
Yinghua TU
;
Zuping LIAN
;
Youke XIE
- Publication Type:Journal Article
- Keywords:
nasopharyngeal neoplasms;
RNA interference;
cell migration assays;
cell adhesion;
cell line,tumor;
blot-ting,Western;
reverse transcriptase polymerase chain reaction;
MTA1;
nasopharyngeal cancer;
5-8F cell
- From:
Tianjin Medical Journal
2014;(4):309-311,401
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of MTA1 knock down on migration and invasion of NPC cell 5-8F in vitro. Methods RNAi (Si-MTA1-01 and Si-MTA1-02) that can transiently silenced MTA1 was designed, synthesized and transfected into 5-8F cells by lipofectamine 2000. Control group (transfection with nonsense sequence) was also estab-lished. The efficiency of MTA1 depletion was determined by q-PCR and Western blot. Wound-healing assay ,Matrigel inva-sion assay and thesolid-phase adhesion assay were performed to investigate the effect of MTA1 knockdown on 5-8F cell me-tastasis. Results Transiently knock down of MTA1 decreased MTA1 transcription and expression in 5-8F cells compared to shRNA-con cells, showing by Real-time PCR and western blot. The invasion and migration of the cells transfected with siRNA-MTA1 were much weaker than the control group (P＜0.05). Conclusion silencing MTA 1 gene can effectively in-hibit the migration and invasion of nasopharyngeal carcinoma cell, and might be a promising target for NPC treatment.
