GATA4 Expression During Induction of the Cardiomyocyte-Like Cells Differentiation Regulated by miR-122
10.3969/j.issn.0253-9896.2014.04.013
- VernacularTitle:miR-122靶向调控心肌样细胞诱导分化过程中GATA4基因的表达
- Author:
Lijing GUO
;
Zhi ZHANG
;
Zidong LIU
;
Wei FU
;
Zhou JI
- Publication Type:Journal Article
- Keywords:
mesenchymal stem cells;
MicroRNAs;
GATA4 transcription factor;
3′untranslated regions;
computation-al biology;
rats,Sprague-Dawley;
miR-122;
cardiomyocyte-like cells
- From:
Tianjin Medical Journal
2014;(4):333-336,404
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify the miR-122 which regulateing GATA4 expression during the induction of rat bone marrow mesenchymal stem cells (bMSCs) differentiating into cardiomyocyte-like cells. Methods BMSCs were isolat-ed from bone marrow and induced to differentiate into cardiomyocyte-like cells using 5-azacytidine. The miR-122 which may regulate expression of GATA4 were predicted using miRanda and TargetScan softwares and identified by dual luciferase report system. The expressions of miR-122 and GATA4 were determined using q-PCR during the differentiation of bMSCs into cardiomyocyte-like cells. Results The induced cells were completely in contacted with adjoining cells and uniform in shape and aligned parallelly. Cardiac troponin I (cTnI) expression was detected by immunofluorescence cytochemistry. Using dual luciferase reporter system in vitro, miR-122 were proved to be able to effectively inhibit GATA4 expression by binding the 3′UTR of GATA4 mRNA. q-PCR results showed that the expression of miR-122 is negatively correlated with that of GATA4 mRNA transcription. Conclusion These results indicated that miR-122 regulate the expression of GATA4 during the induction of cardiomyocyte-like cells.
