Expression of enhancer of zeste homolog 2 in U266 cell line and bone marrow mononuclear cells of multiple myeloma patients

VernacularTitle:多发性骨髓瘤患者原代细胞与U266细胞株zeste基因增强子同源物2表达升高的初步研究
Author: Yanping MA ; Gu JIA ; Ling ZHANG
Publication Type:Journal Article
Keywords: Multiple myeloma; DNA methylation; Enhancer of zeste homolog 2
From: Journal of Leukemia & Lymphoma 2013;22(5):284-285,293
CountryChina
Language:Chinese
Abstract: Objective To investigate the expression of DNA methylation related protein enhancer of zeste homolog 2 (EZH2) in multiple myeloma (MM) cell line U266 and enriched MM cells (MM-BMMNC) from 10 patients,and analyze the potential role of DNA methylation in the occurrence and development of MM.Methods Human MM cell line U266 and MM cells from 10 MM patients were studied.Enriched bone marrow mononuclear cells (N-BMMNC) from 10 healthy persons were used as the control.The expression of EZH2 were determined by Western blot using the ratio of target protein EZH2 and the internal reference β-actin expression as the final results.Results The preliminary in vitro results showed that EZH2 expression was higher in MM cell line U266 and patient MM-BMMNC (N-BMMNC 0.2277±0.1306,MM-BMMNC 0.9220±0.1301,U266 0.9730±0.1029),with statistically significant differences (P ＜ 0.05).Conclusion EZH2 is enriched in U266 and MM-BMMNC.DNA methylation may have a close correlation with the occurrence and development of MM.