Diagnosis value of immunophenotype and karyotypes in the de novo chronic lymphocytic leukemia
10.3760/cma.j.issn.1009-9921.2012.11.011
- VernacularTitle:免疫分型联合核型检查对初诊慢性淋巴细胞白血病的诊断价值
- Author:
Jixia QIN
;
Meiyun FANG
;
Guozhen SUN
;
Hongju PENG
;
Guang SUN
;
Yuejian LIU
;
Yuan JING
;
Feng JIANG
- Publication Type:Journal Article
- Keywords:
Leukemia,lymphocytic,chronic;
Immunophenotyping;
Karyotyping
- From:
Journal of Leukemia & Lymphoma
2012;21(11):681-683,696
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the diagnosis value of immunophenotype and karyotypes in newly diagnosed chronic lymphocytic leukemia (CLL).Methods To retrospect the flow cytometry (FCM) immunophenotype and karyotypes characteristics in newly diagnosed 70 CLL cases.Results In all cases,the positive rates of CD19,CD20,CD5,CD23,CD22 were 100 ％,88.5 ％ (54/61),77.1％ (54/70),67.6 ％ (23/34)and 51.9 ％ (27/52),respectively.And 6 were misdiagnosed,2 was CD+5CD+19(+),but CD20,CD22 were strongly positive,final diagnosed as mantle cell lymphoma (MCL) by FISH t(11;14) examination and CyclinDl; CD+5CD+19(-) CLL were 16 cases (22.9 ％),but 4 were misdiagnosed,the misdiagnosis rate was 25 ％,significantly higher than that of CD+5CD+19(-) CLL (P =0.030).59 cases were examined by conventional cytogcnetic (CC),and 13 cases were with abnormal karyotypes,positive rate was 22.0 ％,with complex karyotypes in 5 cases (8.5 ％); 10 cases combined with FISH abnormalities karyotype examination rate was 60 ％.Conclusion Typical CLL immunophenotypic characteristics were CD5,CD19,CD23 co-expression,and CD－5 CLL with higher misdiagnosis rate,combined with CD20 (,) CD22 expression and karyotype analysis helps to CLL and other B lymphoid proliferative diseases (B-LPD) identification.Conventional cytogenetic detection combined with FISH scan can improve the recognition ability of abnormal chromosome.
