PSF/SFPQ relocated on cell membrane in hematologic neoplasia, a potential MDR target of hematologic tumors
10.3760/cma.j.issn.1009-9921.2012.11.002
- VernacularTitle:富含脯氨酸和谷氨酰胺的剪切因子:预测血液肿瘤耐药的潜在靶点
- Author:
Simei REN
;
Qian LIU
;
Hongwei PENG
;
Yanjun ZHANG
;
Dongsheng XIONG
;
Yizhi ZHANG
- Publication Type:Journal Article
- Keywords:
Multidrug resistance;
PSF;
Hematologic neoplasms;
Target;
Prediction
- From:
Journal of Leukemia & Lymphoma
2012;21(11):646-649,653
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify multidrug resistance (MDR) associated cell surface antigen in hematologic neoplasia and to investigate the universality of membrane-relocated expression of this antigen in hematologic neoplasia.Methods The membrane antigen was isolated and precipitated by SDS-PAGE and co-immunoprecipitation (co-IP),then was identified by mass spectrum (MS).Specific siRNA was used to interfere with gene expression,laser confocal microsopy was used to validate the results involved in antigen information.FACS was performed to analyse relocated expression of the antigen in hematologic neoplasia.Results Co-IP and MS show that a nuclear factor PSF was the antigen of 5D12,a leukemia-MDR associated McAb,and this antigen could relocate on HL-60 cell membrane.A series of experiences further confirmed that PSF overexpressed on HL-60 cell membrane compared with HL-60/ADR.The binding percentages of 5D12 to many hematologic tumor cells were observed,HL-60 (78.56±0.76) ％,K562 (26.54±4.42) ％,Nomalwa (38.10±5.11) ％,U937 (64.03±7.96) ％,Jurkat (29.12±5.58) ％,Raji (74.92±3.41) ％,CEM (12.18±3.21) ％.Conclusion Nuclear protein,PSF relocalizes on cell surfaces in hematologic tumor cells and contributes to cell sensitivity.PSF is a potential target of MDR prediction in hematologic neoplasia.
