Infection results analysis of immunofluorescence method detection of RSV
10.3969/J.ISSN.1672-8270.2015.03.023
- VernacularTitle:间接免疫荧光法检测幼儿呼吸道合胞病毒感染结果分析
- Author:
Chunlan WU
;
Jianfen WU
- Publication Type:Journal Article
- Keywords:
Immunofluorescence method;
Respiratory syncytial virus;
Infection
- From:
China Medical Equipment
2015;(3):70-72,73
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the infection results of The immunofluorescence detection of respiratory syncytial virus (RSV) in huazhou region from 2009 to 2010.Methods: The N gene sequence of RSV was taken as a reference to design specific primers and TaqMan fluorescent probe and to establish real-time The immunofluorescence method to detect sensitivity and specificity.21548 swab samples of children with acute respiratory infections in huazhou region from 2009 to 2010 were tested with real-time fluorescence. The immunofluorescence method.Results: The specificity and sensitivity of real-time fluorescence. The immunofluorescence method for detection of RSV infection were higher, without cross-reactivity to other respiratory viruses.10,477 cases of clinical specimens in 2009 were detected, with positive RSV infection rate of 9.73%, by real-time The immunofluorescence method detection. Positive detection rate of RSV infection had two peaks, which lied in February and December; the positive rate was 11% and 11% respectively. RSV infection rate was the highest for children under 6 years old.Conclusion: Real-time fluorescence features convenient detection, high specificity, and high sensitivity, which can be used for clinical detection of RSV infection. From 2009 to 2010, RSV infection remains a major pathogen of acute respiratory infections in children in huazhou region.
