Effect of PPAR-δ on lung injury of rats induced by hyperbaric oxygen exposure

VernacularTitle:PPAR-δ在高压氧诱导大鼠肺组织损伤中的作用
Author: Fangfang WANG ; Yiqun FANG ; Xiaochen BAO ; Jun MA ; Pu YOU ; Shi ZHANG
Publication Type:Journal Article
Keywords: PPAR-δ; hyperbaric oxygen; pulmonary oxygen toxicity
From: Military Medical Sciences 2014;(8):591-593,601
CountryChina
Language:Chinese
Abstract: Objective To investigate the effect of PPAR-δ on the lung injury of rats induced by hyperbaric oxygen (HBO2) exposure.Methods Sixty male Sprague-Dawley rats were randomly divided into six groups:air+vehicle, air+GW0742, and air+GSK0660, HBO2 +vehicle, HBO2 +GW0742, HBO2 +GSK0660.Lung injury was induced in rats by HBO2exposure (2.3 ATA, 100%O2, 8 h).Rats were injected with vehicle[10%DMSO in 0.3 ml NaCl 0.9%(v/v)] or GW0742 (0.3 mg/kg, ip) or GSK0660 (1 mg/kg, ip) at 1, 6 and 12 hours before either air or oxygen exposure .Protein levels in the bronchoalveolar lavage fluid ( BALF) , wet/dry ratio of the lung and the pathological changes in the lung tissue were detected 30 min after rats′egress.Results and Conclusion For the HBO2 +GW0742 group, the protein levels in BALF, the wet/dry ratio of the lung and the pathological changes in lung tissues all significantly decreased compared with those of the air group .These changes in HBO 2 +GSK0660 group tended to increase the level of lung injury .PPAR-δhas a protective effect on pulmonary oxygen toxicity induced by HBO 2 .