Curcumin enhancing sensitivity of acute myeloid leukemia stem cells to daunorubicin by down-regulating expression of CyclinD1 and Bcl-2
10.3969/j.issn.1671-8348.2015.12.009
- VernacularTitle:姜黄素下调CyclinD1和Bcl-2的表达增强急性髓系LSCs对柔红霉素的敏感性
- Author:
Quanhong WANG
;
Yonghuai FENG
;
Yuhe WANG
- Publication Type:Journal Article
- Keywords:
curcumin;
daunorubicin;
leukemia;
Bcl-2;
CyclinD1
- From:
Chongqing Medicine
2015;44(12):1613-1616,1619
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the potential mechanism of curcumin(CUR) enhancing the sensitivity of acute myeloid leu-kemistem cells(LSCs) CD34+CD38-KG1cellto daunorubicin (DNR) .MethodThe expression of surface moleculeCD34 , CD38 in KG1cellwadetected by the flow cytometry .The half maximal inhibitory concentration (IC50 ) of curcumin to CD34+CD38-Kglcellwacalculated by the Mtmethod .MT,methylcellulose colony formation assay and flow cytometry were ap-plied to examine the effectof Dnon the proliferation ,clone forming ability and apoptosiin the two kindof cell(CD34+CD38-KG1celland CUR/CD34+CD38-KG1cells) respectively .The mRNA-expression of Bcl-2 ,Bax and XIAP in the two cellwere analyzed by RT-PC.Meanwhile ,the protein-expression of CyclinD1 ,Bcl-2 ,Bax and XIAP were detected by Western Blo.ResultThe percentage of CD34+CD38-KG1in KG1cell line wa(98 .2 ± 3 .2)% .The IC50 of curcumin treating CD34+CD38-KG1fo24 h wa100 μmol/L .The inhibition effecof high and middle concentration(0 .8 ,2 .0 μg/mL) of Dnon the proliferation of CUR/CD34+CD38-KG1cellwastrongethan thaof CD34+CD38-KG1cell(P＜0 .05) .The inhibitory role of Dnto the colony formation on CUR/CD34+CD38-KG1cellwamore effective than thaof CD34+ CD38-KG1cells(P＜0 .05) .The ap-optotirate of CUR/CD34+ CD38- KG1cellin each concentration group of Dnwahighethan thaof CD 34+ CD38- KG1cells(P＜0 .05) .The mRNA-expression of Bcl-2 and the protein-expression of Bcl-2 and CyclinD1 were down-regulated .Howeve, no significanchange waobserved aboth mRNA-expression and protein-expression of Bax and XIAP .Conclusion Cucan en-hance the sensitivity of CD34+CD38-KG1cellto DN,which mighbe associated with down-regulating the expression of Cy-clinD1and Bcl-2 .
