Relationship between immune function of T cells in peripheral blood and expression of hepatitis C virus in patients with chronic hepatitis C
10.3969/j.issn.1671-8348.2015.12.011
- VernacularTitle:CHC患者外周血T细胞免疫功能与丙型肝炎病毒表达的关系
- Author:
Qunying LI
;
Mingli LIANG
;
Li CHEN
;
Lijuan WU
;
Yugang LIU
;
Tong LIU
;
Jing XIE
;
Zonghai HU
;
Yuanqing QU
- Publication Type:Journal Article
- Keywords:
hepatitis C;
T-lymphocyte subsets;
flow cytometry;
polymerase chain reaction
- From:
Chongqing Medicine
2015;44(12):1620-1622,1625
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the relationship between the immune function of cellin peripheral blood with the virureplication and hepatitiviru(HCV)-cAg expression in the patientwith chronihepatiti(CHC) .MethodPeripheral blood lymphocytesubpopulation ,HCV-Rnand HCV core antigen (HCV-cAg) in 63 healthy people undergoing the physical exami-nation (control group) and 85 caseof CHC(Chgroup) were analyzed by the flow cytometry ,real-time Pcand ELIS,respec-tively .ResultThe percentageof total cell,T4 cell,T8 cell,double negative cell(DN) and double positive cell(DP) in the Chgroup were (67 .37 ± 10 .43)% ,(37 .11 ± 10 .28)% ,(21 .63 ± 8 .87)% ,(7 .80 ± 4 .57)% and (0 .20 ± 0 .29)% , respectively ,the absolute contentwere in turn (0 .70 ± 0 .44) × 109/L ,(0 .37 ± 0 .22) × 109/L ,(0 .22 ± 0 .17) × 109/L ,(0 .08 ± 0.06)×109/Land(0.19±0.68)×107/L,respectively.TheratioofT4/T8was(2.18±1.26)% .Theresultsindicatedthatthe percentage of T8 cellin the Chpatientwadecreased obviously (P＜0 .01) ,which resulted in the ratio of T4/T8 raising(P＜0 .05);meanwhile ,the absolute contentof the total cell,T4 cell,T8 celland Dnwere all decreased obviously (P＜0 .05);moreove,the percentage of T4 celland Dnin the patientwith HCV-Rnpositive and HCV-cAg positive wasignificantly in-creased (P＜0 .05) .Conclusion When HCV replicating in the patientwith CHC,the T lymphocyte subpopulation haobviouab-normity .The low immune function or immune tolerance ofT cells may be the important cause of recurrence and uncurability of CHC.
