Antibody titre quantitation method by ELISA without standard substance based on kinetics of enzyme-catalyzed reactions
10.3969/j.issn.1671-8348.2015.12.026
- VernacularTitle:基于酶促反应动力学的无标准品ELISA抗体滴度定量方法
- Author:
Cheng FANG
;
Zhuo CHEN
;
Li LIU
;
Zhiqiang MEI
- Publication Type:Journal Article
- Keywords:
kinetics of enzyme-catalyzed reactions;
enzyme-linked immunosorbent assay;
antibody titre
- From:
Chongqing Medicine
2015;44(12):1662-1663,1666
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish antibody titre quantitation method by ELISwithoustandard substance .MethodThe tesgroupof non-specifi,negative control ,specifiand total antibodiewere sefodetecting serantibodieby ELIS.Aftelineafitting of the time-absorbance datof early developing ,the fitted slopewere used athe velocity of absorbance changing , which were denoted by ν0 ,νC,νS,νT.Based on thathe ν-value and the concentration ? of determinand were linearelationship while the substrate waexcessive ,the function with parameteof ν-valueforeflecting the multiple proportionof antibodiecon-centrationbetween specifiand negative control groupcould be deduced .The assessmenof specifiIgG antibodiein serof KM mice immunized with fish collagen waused aan instance .ResultThe function C/C= (ν-ν0 )/(νC-ν0 ) could calculate the mul-tiple proportion(titre) of antibodieconcentrationbetween specifiand negative control group.Conclusion The above method of antibody titre quantitation isuitable fosemi-quantitative analysiwithoustandard substance .
