A Study on Dermatophagoides pteronyssinus Induced Cytokines Gene Expression in Peripheral Blood Mononuclear Cells.
- Author:
Kyong Og KO
1
;
Dul Le MIN
;
Jung Kyu PARK
;
Young Hun CHUNG
Author Information
1. Department of Pediatrics, College of Medicine, Konyang University, Korea.
- Publication Type:Original Article
- Keywords:
Cytokines;
Th2 response;
IL-4;
IL-5;
Asthmati
- MeSH:
Adult;
Asthma;
Child;
Clone Cells;
Cytokines*;
Dermatophagoides pteronyssinus*;
Gene Expression*;
Humans;
Interleukin-2;
Interleukin-4;
Interleukin-5;
Lymphocytes;
Negotiating;
Phenotype;
Pyroglyphidae*;
RNA, Messenger
- From:Pediatric Allergy and Respiratory Disease
2001;11(2):109-121
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Cytokines are known to play a major role in mediating many of the immunological and pathological findings of allergic disease. Many studies on allergen-specific T cell clones isolated from atopic individuals have shown that these cells produce cytokines of the Th2 phenotype. The purpose of the present study was to investigate cytokine patterns of T lymphocyte induced by Dermatophagoides pteronyssinus in atopic asthma. METHODS: Freshly isolated peripheral blood mononuclear cells of Dp(+) asthmatic children, Dp(-) asthmatic children, nonasthmatic children, and normal adults were stimulated with Dp antigen and examined for the induction of IFN-gamma, IL-2, IL-4, and IL-5 mRNA using RT-PCR. RESULTS: The lymphoproliferative response to crude Dp antigen was maximized at Dp concentration of 2.5 microgram/mL. The lymphoproliferative response was low in children including Dp(+) asthmatics, Dp(-) asthmatics and nonasthmatics, but that of normal adults was high. The expression of IFN-gamma was not so different in each group, but the expression of IL-4 was least in adults, and that of IL-5 was most prominent in Dp(+) asthmatic children. In adult healthy group, IFN-gamma expression without IL-4 and IFN-gamma without IL-5 was significantly higher than those in children. IL-5 expression with or without IFN-gamma was the highest in Dp(+) asthmatic children. IL-4 and IL-5 or IL-5 without IL-4 expression were observed more frequently in Dp(+) asthmatic children than in any other groups. Th2 response was most prominent in Dp(+) asthmatics, and Th1 response was most prominent in normal adult group. CONCLUSION: In conclusion, we observed significantly prominent Th2 response in Dp(+) asthmatic children, especially that of IL-5.
