Study on the mechanism of S100A9-induced secretion of vascular endothelial growth factor-A by monocytes
10.3969/j.issn.1673-4130.2015.13.006
- VernacularTitle:S100A9诱导单核细胞分泌血管内皮生长因子-A 的初步机制研究?
- Author:
Chunlong LI
;
Hong LIAO
;
Ming JIANG
- Publication Type:Journal Article
- Keywords:
S100A9 protein;
vascular endothelial growth factor-A;
the receptor for advanced glycation endproducts;
NK-κB signal
- From:
International Journal of Laboratory Medicine
2015;(13):1816-1817,1820
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the molecular mechanism of S100A9-induced secretion of vascular endothelial growth factor-A (VEGF-A)by monocytes.Methods Peripheral blood specimen were collected from healthy individuals undergoing physical exami-nation and the CD14 + monocytes were purified by using immunomagnetic beads and the expression of the receptor for advanced gly-cation endproducts (RAGE)was detected by flow cytomertry.In vitro CD14 + monocytes were stimulated by S100A9,and anti-RAGE antibody or NK-κB signal pathway inhibitor were pre-incubated for 1 hour and then stimulated by S100A9,the levels of VEGF-A were detected by using enzyme-linked immunosorbent assay.Results The high level of RAGE was expressed by isolated CD14 + monocytes,after S100A9 stimulation,the secretion of VEGF-A by CD14 + monocytes was significantly increased in a dose and time dependent manner.However,the inducing VEGF-A was significantly decreased(P <0.01 ),while pre-treated with anti-RAGE antibody or NK-κB inhibitor (P <0.01).Conclusion S100A9 inducing the secretion of VEGF-A by monocytes and is de-pended on RAGE-NK-κB signal pathway,suggesting that S100A9 might promote angiogenesis.
