Analysis of the expression of AFP-L3 and GPC-3 in primary hepatic carcinoma with different concentration of AFP
10.3969/j.issn.1673-4130.2015.13.020
- VernacularTitle:不同 AFP 浓度下 AFP-L3与 GPC-3在原发性肝癌中的表达分析
- Author:
Wenyan QIN
;
Bo CHEN
;
Zisheng LI
;
Youqing SHE
- Publication Type:Journal Article
- Keywords:
primary hepatic carcinoma;
alpha-fetoprotein;
alpha-fetoprotein variant 3;
glypican-3
- From:
International Journal of Laboratory Medicine
2015;(13):1849-1850,1853
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyse the expression of alpha-fetoprotein variant-L3(AFP-L3)and glypican-3(GPC-3)in primary he-patic carcinoma(PHC)with different concentration of alpha-fetoprotein (AFP),so as to provide references for the diagnosis of PHC.Methods 240 cases of outpatients,inpatients and individuals on physical examination were selected as subjects and serum levels of AFP-L3 and GPC-3 were detected.All of the subjects were divided into negative group(0≤AFP<20 ng/mL),low concen-tration group(20≤AFP<400 ng/mL)and high concentration group(AFP≥400 ng/mL)according to the serum levels of AFP.Ser-um levels of AFP-L3 and GPC-3 were compared among the three groups.And the sensitivity,specificity and accuracy of single de-tection of AFP-L3 or GPC-3 and those of combined detection of AFP-L3 and GPC-3 were compared as well.Results The serum levels of AFP-L3 and GPC-3 in the low concentration group and high concentration group were both higher than those in the nega-tive group,and those in the high concentration group were also higher than those in the low concentration group,had statistically significant differences(P < 0.05 ).The sensitivity,specificity and accuracy of combined detection of AFP-L3 and GPC-3 were 84.4%,95.9% and 93.8% respectively,which were higher than those of single detection of AFP-L3 or GPC-3.Conclusion Com-bined detection of AFP-L3 and GPC-3 could improve the sensitivity,specificity and accuracy for diagnosis of PHC,which has clinical significance for the diagnosis of PHC.
