Effects of zonisamide on NO content and NOS activity in serum and brain tissue of epileptic rats
10.11958/j.issn.0253-9896.2015.05.007
- VernacularTitle:唑尼沙胺对癫痫大鼠血清及脑组织NO含量及NOS活性的影响
- Author:
Fang CHEN
;
Bin LIU
;
Jing ZHANG
;
Wei WANG
;
Suzhen SUN
;
Lihui WANG
- Publication Type:Journal Article
- Keywords:
anticonvulsants;
epilepsy;
pentylenetetrazole;
nitric oxide;
nitric oxide synthase;
malondialdehyde;
superoxide dismutase;
rats;
Sprague-Dawley;
zonisamide
- From:
Tianjin Medical Journal
2015;43(5):474-476,579
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of zonisamide as a new antiepileptic drug on nitric oxide (NO) content and nitric oxide synthase (NOS) activity in serum and brain tissue of epileptic rats. Methods Eight healthy rats were used as normal control group, and twenty-four epileptic rats induced by pentrazol were randomly divided into epilepsy model group, zonisamide group and phenobarbital group. Levels of NO and malondialdehyde (MDA) content, NOS and superoxide dismutase (SOD) activity in serum and brain tissue were detected in four groups. Results Forty-two rats were injected pentrazol, and 35 (83%) rats were established the rat model successfully. Epileptic waves were visible in EEG of epileptic rats. The concentrations of NO, MDA and the activity of NOS in serum and brain were significantly increased, the activity of SOD was significantly decreased, in epileptic rats than those of control rats. The concentrations of NO and MDA were significantly increased; the activity of SOD was significantly decreased, in brain in phenobarbital group compared with those of control group. There were significantly lower levels of NO, MDA and NOS, and significantly higher level of SOD in serum and brain tissue in zonisamide group and phenobarbital group than those of epileptic model group (P＜0.05). Conclusion Zonisamide plays an antiepileptic role by reducing the concentration of NO in brain of epileptic rats.
