Detection of Human Hepatocarcinoma Cell Line PLC/PRF/5 Genome Hepatitis b Virus DNA Integration with Alu-PCR
10.11969/j.issn.1673-548X.2015.04.023
- VernacularTitle:Alu-PCR检测人肝癌细胞株PLC/PRF/5基因组中乙型肝炎病毒DNA的整合
- Author:
Shuang QIU
;
Huiying ZHANG
- Publication Type:Journal Article
- Keywords:
Hepatocarcinoma;
Alu-PCR;
Hepatitis B virus DNA;
Integration
- From:
Journal of Medical Research
2015;44(4):88-90,41
- CountryChina
- Language:Chinese
-
Abstract:
Objective In this research with the method of Alu -PCR we investigate the integration of hepatitis B virus ( HBV) DNA in human hepatocarcinoma cell line (PLC/PRF/5) genome DNA.Methods We at first extracted the genome DNA from PLC/PRF/5 cells, and then the potential integration fragments of HBV DNA and human genome DNA were amplified with according to the Alu -PCR after three rounds PCR .The Alu-PCR amplification products were observated with agarose gel electrophoresis , then integration positive electrophoresis bandings were chipped and purified for nucleic acid sequencing .At last he bioinformatics information was acquired by blast online.Results Through agarose gel electrophoresis after Alu -PCR amplification, we got four potential integration bindings , among which we got three integration sequences of HBV DNA in human genome DNA .These integration sequences could be individually located in the human chromosome of 03p21.31, 05p15.33, 12q13 and 12-q14.1.Conclusion With Alu-PCR we can accurately measure the integration of HBV DNA in human genome DNA , and Alu-PCR can be a a convenience and economic method in the study of HBV DNA ′s integration in human genome DNA .
