Study of sanfukechuangao acupoint application for treatment of asthma in guinea pigs
10.11958/j.issn.0253-9896.2015.05.008
- VernacularTitle:三伏咳喘膏治疗支气管哮喘的实验研究
- Author:
Xiangming XIONG
- Publication Type:Journal Article
- Keywords:
sanfukechuangao;
acupoint application;
interleukin-18;
eosnophils;
asthma;
guinea pigs
- From:
Tianjin Medical Journal
2015;43(5):477-479,578
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of sanfukechuangao acupoint application on changes of interleukin-18 (IL-18), eosinophils (EOS) and pulmonary pathology in asthma model of guinea pigs, and its underlying mechanism thereof. Methods A total of 60 guinea pigs were randomized into normal control group, asthma model group, acupoint-application 1 h group, acupoint-application 3 h group, acupoint-application 6 h group and dexamethasone group. Asthma model was established by intraperitoneal injection of 10%ovalbumin (OVA,1 mL) and forced inhalation of atomized 2%OVA. Skin preparation and external application of Chinese medicinal herbs were made on back of guinea pigs, and Feishu,Xinshu,Geshuwere selected in acupuncture points). Levels of IL-18 and EOS were detected by immunohistochemisty method. Results Compared with model group,the levels of IL-18 and EOS were significantly decreased, the pathological changes were significantly reduced in three acupoint-application groups. The effectiveness of acupoint-application 3 h group was the best in three groups. Conclusion Sanfukechuangao acupoint application can influence the levels of IL-18 and EOS effectively, and relieve phlegmonosis injuries in pulmonary tissue of asthma model of guinea pig.
