Study of Reversing Invasion of Human Gastric Cancer Cell Line BGC-823 by Targeting Angiopoietin-1 Using siRNA
10.3969/j.issn.0253-9896.2014.08.005
- VernacularTitle:靶向Ang-1的siRNA片段逆转人胃腺癌细胞株BGC-823侵袭表型的研究
- Author:
Yuxin XING
;
Wei WANG
;
Xianmin SANG
;
Yuanshun ZHAO
;
Chunze ZHANG
- Publication Type:Journal Article
- Keywords:
angiopoietin-1;
RNA interference;
stomach neoplasms;
neoplasm invasiveness;
antigens,CD29;
antigens,CD44
- From:
Tianjin Medical Journal
2014;(8):752-754,849
- CountryChina
- Language:Chinese
-
Abstract:
Objective To knock down angiopoietin-1 expression in human gastric cancer cell line BGC-823 and to observe its effect of reversing tumor invasion. Methods siRNA sequence fragments was designed to target angiopoietin-1 and transferred into human gastric cancer cell line BGC-823. RT-PCR was used to assess the transcription level of angio-poietin-1 mRNA, then western blot and immunofluorescence were used to examine the expression level of three invasion-as-sociated proteins include integrinβ1, CD44V6 and Ang-1. Cell adhesion ability was evaluated by cell adhesion assay and cell invation was determined by matrigel and transwell plastic dual-chamber culture system. Results Ang-1 mRNA was knocked down by siRNA showed by RT-PCR. The expression of integrinβ1, CD44V6 and Ang-1 were significantly lower than control group(P<0.05), so did the cellular adhesion and invasion abilities(P<0.05). Conclusion Knocking down angiopoietin-1 by siRNA can reverse invasion of human gastric cancer cell line BGC-823 and may provide new ideas and reference for gene therapy of gastric cancer in the future.
