Effects of Propofol on Triggering Receptor Expressed on Myeloid Cells-1 (TREM-1) by Lipopolysaccharide-Stimulated Human Monocyte Cell Lines
10.3969/j.issn.0253-9896.2014.08.007
- VernacularTitle:异丙酚对内毒素诱导人单核细胞系THP-1细胞TREM-1表达的影响
- Author:
Liren LU
;
Liangqing ZHANG
;
Yan LU
- Publication Type:Journal Article
- Keywords:
endotoxins;
lipopolysaccharides;
propofol;
monocytes;
tumor necrosis factor-alpha;
triggering receptor expressed on myeloid cells 1
- From:
Tianjin Medical Journal
2014;(8):759-761,785
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effects of propofol on TREM-1 expression in THP-1 cells stimulated by lipo-polysaccharide (LPS). Methods Cells were divided into 7 groups: (1) control group; (2) LPS group that was exposed to LPS in final concentration of 100μg/L;(3) Progroup that was incubated with 100μmol/L propofol;(4-7) groups combined 100μg/L LPS with propofol at different dose of 12.5μmol/L (P1 group), 25μmol/L (P2 group), 50μmol/L (P3 group), 100μmol/L (P4 group) respectively. After 16 hours of incubation, the TNF-α concentration in supernatants were measured by ELISA. TREM-1 expression were examined by FACS and TREM-1 mRNA were detected using qRT-PCR. Results TREM-1 on THP-1 increased significantly in group P3 and group P4 compared with LPS group (P<0.05). The concentra-tions of TNF-αin P3 and P4 (P<0.05) decreased substantially in supernatant compared with LPS group. TREM-1 mRNA transcription level in group LPS rise considerably (P < 0.05) compared to that in control group, and it dropped greatly in group P4 (P <0.05) compared with that in group LPS. Conclusion Propofol could enhance TREM-1 expression on sur-face of THP-1 stimulated by LPS. Propofol reduces TNF-αlevel in culture supernatant. And propofol may restrain TREM-1 mRNA expression.
