Effects and expression of KLF4 in leukemic cell line K562
10.3760/cma.j.issn.1009-9921.2009.03.004
- VernacularTitle:锌指蛋白KLF4在K562细胞株中的表达和作用
- Author:
Ruijuan ZHANG
;
Linhua YANG
- Publication Type:Journal Article
- Keywords:
Proteins;
Transcription factors;
Leukemia;
K562 cells
- From:
Journal of Leukemia & Lymphoma
2009;18(3):137-138,145
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression and effect of zinc-finger protein kuppel-like factor4(KLF4) in leukemic cell line K562. Methods The lever of mRNA and protein of KLF4 is determined with real-time PCR and Western blot in human peripheral monoeyte/macrophage and K562 cells. Results Compared with control group (0.43±0.03), the expression of KLF4 mRNA in K562 cells (0.02±0.01) wassignificant different(P＜0,05). The expression of KLF4 protein in human peripheral monocyte macrophage is higher than that in K562 cells. Conclusion The low expression of KLF4 was present in leukemic cell line K562,which may suggest that KLF4 gene maybe a tumor inhibitor factor, and partially involved in leukemogenesis.
