Drugs Interference in Microcirculatory Patterns of Transplant Melanoma in Embryonic Chicken Model
10.3969/j.issn.0253-9896.2009.10.012
- VernacularTitle:鸡胚尿囊膜黑色素移植瘤模型中微循环模式药物干预观察
- Author:
Hong QI
;
Xiulan ZHAO
;
Shiwu ZHANG
;
Chunsheng NI
;
Danfang ZHANG
;
Wenzhi ZHANG
;
Baocun SUN
- Publication Type:Journal Article
- Keywords:
melanoma;
neoplasm transplantation;
neovascularization;
pathologic;
endostatins;
doxycycline;
disease models;
animal
- From:
Tianjin Medical Journal
2009;37(10):853-855,后插4
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the effects of endostatin and doxycycline on microcirculatory patterns in the melanoma transplant model of the chorioallantoic membrane of the chicken embryo and the experimental evidences for melanoma therapy thereof. Methods: Endostatin was dripped on the chorioallantoic membrane after melanoma was transplanted 3 days (A group) and 5 days (B group). Doxycycline was dripped on the chorioallantoic membrane after melanoma was transplanted 3 days (C group) and 5 days (D group). The PBS solution was dripped on the chorioallantoic membrane in control group (E group). The hematoxylin-eosin（HE）staining and immunohistochemical staining were performed to observe microcirculation patterns in sections. Results: Compared with the E group, the area of endothelium-dependent vessels were significantly decreased in A group and B group (P ＜ 0.01). But the areas of vasculogenic mimicry were bigger in the two groups(P ＜ 0.05). Compared with E group, the areas of endothelium-dependent vessels and vasculogenic mimicry were significantly decreased in D group(P ＜ 0.05). Conclusion: Endostatin can inhibit angiogenesis. But it has no effect on vasculogenic mimicry in the transplant melanoma. And Doxycycline can inhibit vasculogenic mimicry and angiogenesis in the transplant melanoma. The results provided experimental evidences for melanoma therapy.
