Primary study of the phenotype and biological characteristics of chronic myelogenous leukemia derived mesenchymal stem cells
10.3760/cma.j.issn.1009-9921.2008.02.003
- VernacularTitle:慢性粒细胞白血病患者骨髓来源间充质干细胞生物学特性的研究
- Author:
Chunmei ZHENG
;
Feng LIU
;
Rou MA
;
Liu LI
;
Naiping HU
;
Xiaomei HU
;
Yonggang XU
;
Xiaohong YANG
;
Hongzhi WANG
;
Chi LIU
;
Shanshan ZHANG
;
Haiyan XIAO
- Publication Type:Journal Article
- Keywords:
Leukemia,myeloid,chronic;
Mesenchymal stem cell;
Fusion proteins,bcr-abl
- From:
Journal of Leukemia & Lymphoma
2008;17(2):88-90,97
- CountryChina
- Language:Chinese
-
Abstract:
Objective To isolate and study the phenotype and biological characteristics of chronic myelogenous leukemia-derived mesenchymal stem cells. Methods Cultivate the mesenchymal stem cells from chronic myelogenous leukemia in vitro, identify the immunophenotype by fluorescence-activated cell sorter (FACS), and detect the bcr-abl fusion gene by RT-PCR and FISH. Results The mesenchymal stem cells from chronic myelogenous leukemia can express the bcr-abl fusion gene. Conclusion These findings provide the evidence that the rearrangement of bcr-abl gene might happen at least more primitive than CD+34 CD-38 cells.
