Comparison of methods of natural killer cell sorting and purification from human peripheral blood
10.3760/cma.j.issn.1009-9921.2011.03.005
- VernacularTitle:外周血中自然杀伤细胞分选纯化方法的比较
- Author:
Jing YANG
;
Liping SU
;
Xianxia MAI
;
Wen SU
;
Yanfeng WANG
- Publication Type:Journal Article
- Keywords:
Flow cytometry;
Magnetic activated cell sorting;
Killer cells,natural
- From:
Journal of Leukemia & Lymphoma
2011;20(3):144-146,153
- CountryChina
- Language:Chinese
-
Abstract:
Objective To apply fluorescence-activated cell sorting (FACS) and magnetic activated cell sorting (MACS) in sorting natural killer (NK) cells from human peripheral blood. The efficiency of these two methods were compared so that the basic conditions for the further study on the lower proportion of peripheral blood cells could be created. Methods Twenty cases of normal peripheral blood and 3 cases of post-hematopoietic stem cell transplantation (post-HSCT) were collected and NK cell were sorted by FACS and MACS. Results Before sorting the normal peripheral blood, the proportion of the NK cell in the leukocyte was (12.86±3.62) %, the purity of NK cell was up to (96.15±2.03) % and the recovery was (95.08±2.16) %after sorting by FACS, the purity was up to (93.35±3.61) % and the recovery was (94.11±3.01) % by MACS.And before sorting the post-HSCT peripheral blood, the proportion of the NK cell in the leukocyte was (11.01±2.08) %, the purity of NK cell was up to (96.22±2.16) % and the recovery was (95.27±1.18) % after sorting by FACS. The purity was up to (90.98±1.94) % and the recovery was (94.54±3.52) % by MACS. Thereis difference in purity of NK cell (t = 5.925, P ＜0.05), while no difference in rate of recovery between 2methods (t = 0.789, P ＞0.05). Conclusion FACS is more efficient, rapidly and easily than MACS in sorting NK cell, especially for post-HSCT cases.
