Studies on the expression of Survivin in human leukemia K562 treated by cytarabine

VernacularTitle:阿糖胞苷对白血病细胞K562凋亡抑制基因Survivin的作用研究
Author: Qian DING ; Xiangzhong ZHANG ; Xueyun ZHONG ; Yunxian CHEN
Publication Type:Journal Article
Keywords: K562 cells; Cytarabine; Genes,suppressor; Drug resistance,neoplasm
From: Journal of Leukemia & Lymphoma 2009;18(5):264-266,273
CountryChina
Language:Chinese
Abstract: Objective To investigate the influences of cytarabine on Survivin gene expression in human leukemia K562 cell line and discuss the mechanism of drug resistance in chemotherapy. Methods The IC50 of cytarabine was chosen by MTr assay. The K562 cells were exposed to certain concentration of cytarabine for about 24 hours and 48 hours. The expression levels of Survivin gene were detected by RT-PCR and Western-blot. Results After exposed to cytarabine for 24 hours and 48 hours, the Survivin mRNA level of K562 cells was significantly elevated about 1.92 and 3.38-fold, and the protein expression level was elevated about 1.92 and 2.64-fold. Conclusion An elevated expression of Survivin was tested in K562 cells treated by cytarabine. Consequently, the elevated expression of Survivin could resist apoptosis induced by chemotherapeutic agent which probably associated with chemotherapeutic drug resistance.