Clinical significance of detection of bcr-abl mRNA in chronic myeloid leukemia
10.3760/cma.j.issn.1009-9921.2011.06.003
- VernacularTitle:bcr-abl mRNA检测在慢性粒细胞白血病诊治中的意义
- Author:
Xiaodong Lü
;
Ruihua FAN
;
Jieying HU
;
Benling XU
;
Xuhua ZHANG
;
Yunfeng SONG
;
Yongping SONG
- Publication Type:Journal Article
- Keywords:
Leukemia,myeloid,chronic;
Real-time polymerase chain reaction;
bcr-abl mRNA
- From:
Journal of Leukemia & Lymphoma
2011;20(6):345-346,349
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate significance of the quantification of bcr-abl mRNA in diagnosis and therapy of chronic myeloid leukemia (CML),essentiality significance for monitoring minimal residual disease. Methods Bcr-abl mRNA of 518 CML patients were detected using real-time PCR. Results Expression of bcr-abl mRNA was gradually increased among blastic phase (BP) (12.6 %),accelerated phase (AP) (25.4 %) and chronic phase (CP) (57.2 %) (P<0.05). Quantification of bcr-abl mRNA was cut down gradually after allotransplantation in the patients and becomes normal after treatment for 6 months. But quantification of bcr-abl mRNA inpatients treated with imatinib mesylate became normal after 12 months. Conclusion Real-time PCR was reliable and can be used for diagnosis,monitoring the treatment outcome,detecting the minimal residual disease,and predicting blast crisis.