Anti-proliferation effect of peroxisome proliferator-activated receptor γ agonist on Raji cells and its mechanism
10.3760/cma.j.issn.1009-9921.2008.05.004
- VernacularTitle:过氧化物酶体增生物激活受体γ激动剂对Raji细胞的增生抑制作用及其机制
- Author:
Jiajun LIU
;
Dongjun LIN
;
Ruozhi XIAO
;
Xianglin PAN
;
Chunyan CHEN
- Publication Type:Journal Article
- Keywords:
Leukemia;
PPARγ;
Apoptosis;
Raji cell
- From:
Journal of Leukemia & Lymphoma
2008;17(5):328-330,333
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the anti-proliferation effect of peroxisome proliferator-activated receptor γ(PPARγ)agonist troglitazone(TGZ)on leukemic Raji cells and its mechanisms.Methods Raji cells in culture medium in vitro were given different concentrations of TGZ(0～60 μmol/L)for 24,48 and 72 h.The inhibitory rates of the cells were measured by MTT assay,cell apoptotic rate was detected by flow cytometry(FCM),agarose gel electrophoresis was used to observe the DNA ladder,and western blotting was used to analyzed the variation of apoptosis related proteins bcl-2,Bax and Survivin.Results TGZ(over 20 μmol/L)could inhibit the growth of Raji cells and cause apoptosis remarkably,the suppression was both in time-and dose-dependent manner.DNA ladder was observed after the cells treated by TGZ for 72 h,and western blotting analysis revealed that anti-apoptotie proteins Survivin and bcl-2 were decreased remarkably while pro-apoptotic protein Bax increased significantly after the cells were treated by TGZ for 48 h.Conclusion PPARγ agonist TGZ can inhibit the growth and induce apoptosis on Raji cells significantly,downregnlating the expression of Survivin and bcl-2 as well as upregulating of Bax expression of Raji cells may be one of its most important mechanisms.
