Experimental Rabbit Model of Hepatic Cirrhosis Induced by Partial Bile Duct Obstruction
10.3969/j.issn.0253-9896.2009.10.020
- VernacularTitle:兔胆总管部分结扎肝硬化模型的建立
- Author:
Shanfeng FU
;
Wensheng CHEN
;
Bo JIN
;
Tao SUN
;
Yingxiang YANG
;
Ping LU
;
Min LIU
;
Lihong CUI
- Publication Type:Journal Article
- Keywords:
liver cirrhosis;
biliary;
bile ducts;
intrahepatic;
ligation;
disease models;
animal;
rabbits
- From:
Tianjin Medical Journal
2009;37(10):878-880,后插5
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To build up rabbit models of hepatic cirrhosis in through common bile duct partial ligation (CBDPL). Methods: Male New Zealand rabbits were subjected to CBDPL to induce hepatic cirrhosis. The liver biopsies were performed during the surgery and after sacrifice to evaluate hepatic fibrosis. The serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin (TBIL), total protein (TP) and albumin (ALB) were determined at week 0, 1, 2, 4 and 11 following the occlusion. Results: There were two kinds of morphological and histological manifestations after CBDPL, the one was inconspicuous hepatic fibrosis coexisted with sacculation-like bile ductal dilation and muddy stone; the other is remarkable hepatic fibrosis and cirrhosis along with uneven bile ductal dilation. The serum levels of ALT, AST, TBIL and DBIL were significantly higher after one week of operation. The levels of ALT, AST, TP and ALB decreased after two-week of operation, and the levels of TBIL and DBIL were returned to the normal level after two-week of operation. Conclusion: The animal models of hepatic cirrhosis can be built up through CBDPL in rabbits.
