The Growth Inhibition Induced by SUMO-1 siRNA in Hepatocellular Carcinoma Cell Line SMMC-7721
10.3969/j.issn.0253-9896.2010.01.002
- VernacularTitle:SUMO-1基因siRNA抑制肝癌细胞SMMC-7721生长的研究
- Author:
Wuhua GUO
;
Lihua YUAN
;
Zhihua XIAO
;
Liangping LUO
;
Jixiang ZHANG
- Publication Type:Journal Article
- Keywords:
liver neoplasms;
carcinoma;
cell line;
tumor;
RNA;
small interfering;
gene silencing
- From:
Tianjin Medical Journal
2010;38(1):4-6,后插1
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the efficiency of silencing small ubiquitin-like modifier-1(SUMO-1) induced by siRNA in hepatocellular carcinoma cell line SMMC-7721 and the growth inhibition of SMMC-7721 thereof. Methods: The SUMO-1 siRNA was transfected into SMMC-7721 by means of lipofectamine~(TM) 2000. The silencing efficiency of SUMO-1 was examined by RT-PCR and western blot. The cell growth and cell cycle were examined by MTT and flow cytometry(FCM). The cell apoptosis was detected by DeadEnd~(TM) Colorimetric TUNEL System. Results: The siRNA could significantly silence the expression of SUMO-1 in SMMC-7721.The maximal silencing rate was utmost 73.43% at 48 hours after being transfected SUMO-1 siRNA. MTT assay revealed that the cell line grew more slowly. FCM result showed that the number of G_2 stage cells was increased significantly. But apoptosis cells were not found by TUNNEL assay. Conclusion: SiRNA is a good manner to silence the expression of SUMO-1 in SMMC-7721 in vitro. Owing to the growth inhibition induced by SUMO-1 siRNA, SUMO-1 plays an important role in development of SMMC-7721.
