Study of the expression of the cyclin D1 in chronic myelogenous leukemia
10.3760/cma.j.issn.1009-9921.2008.03.008
- VernacularTitle:慢性粒细胞性白血病细胞周期蛋白D1基因表达及临床研究
- Author:
Liefen YIN
;
Ling YANG
;
Mengmeng CUI
;
Zhenglei SHEN
;
Xiaoyuan QU
;
Lifang CHENG
;
Guiyun HUANG
- Publication Type:Journal Article
- Keywords:
Leukemia,myeloid,chronic;
Cyclin Dl;
Real-time quantitative polymerase chain reaction
- From:
Journal of Leukemia & Lymphoma
2008;17(3):187-188,191
- CountryChina
- Language:Chinese
-
Abstract:
Objective To examine the expression levels of cyclin Dl in the patients with chronic myelogenous leukemia (CML), and evaluate the pathogenesis and clinical significance of cyclin Dl in CML Methods The real-time quantitative polymerase chain reaction (RQ-PCR) was performed to detect the expression levels of cyclin Dl in the bone marrow samples of 18 patients with CML, and 16 samples of benign hemopoietic patients. The relationship between the expression levels of cyclin Dl and the progression and prognosis of patients with CML were analyzed. Results The level of cyclin Dl was higher expressed in 18 patients with CML than the control group (P <0.001). The levels of cyclin Dl was apparently higher expressed in accelerated phase /blast crisis phase than in chronic phase (P <0.05). And the RQ-PCR method showed the tendency that a significant increase was observed in the levels of cyclin Dl from 0.1980 in control group to 1.4002 in chronic phase and 5.4540 in accelerated phase /blast crisis phase. Conclusion The cyclin Dl overexpressed in CML, the roles of cyclin Dl in CML might be an oncogene expressed. The expression level is correlated with the progression and prognosis of patients with CML.
