Construction and Characteristic of Human Multidrug-Resistant Hepatocellular Carcinoma Cell Line
10.3969/j.issn.0253-9896.2009.10.018
- VernacularTitle:人肝癌耐药细胞系的建立及生物学特性的研究
- Author:
Chao MENG
;
Xiaomin SHEN
- Publication Type:Journal Article
- Keywords:
liver neoplasms;
carcinoma;
drug tolerance;
tumor cells;
cultured;
cell line;
tumor
- From:
Tianjin Medical Journal
2009;37(10):872-873,后插2
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish human multidrug-resistant hepatocellular carcinoma cell line(QGY7703/DDP) to analyze its different resistance and sensitivity. Methods: The human hepatocellular carcinoma cell line QGY7703 was continuously grown in the presence of cisplatin（DDP) for up to 6 months. Cell proliferation and cytotoxicity were serially monitored. Results: The chemosensitivities to 5-FU，DDP，adramycin (ADM) and mitomycin(MMC) were different between QGY7703 and QGY7703/DDP. The exhibitions were 1.533 fold to 5－FU，2.181 fold to DDP，7.08 fold to ADM, 9.461 fold to MMC in QGY7703/DDP. Conclusion: The drug resistance in cancer cell lines can be established by exposing to antitumor drugs in cell culture system.
