Silence Effect of Short Hairpin RNA on Expression of PIM-1 in Prostate Cancer Cell Lines
10.3969/j.issn.0253-9896.2009.07.001
- VernacularTitle:短发夹RNA对前列腺癌细胞中PIM-1基因沉默效应的研究
- Author:
Xiaoguang ZHANG
;
Yong XU
;
Zhihong ZHANG
;
Kuo YANG
;
Baomin QIAO
;
Changyi QUAN
- Publication Type:Journal Article
- Keywords:
prostatic neoplasms;
carcinoma;
cell line,tumor;
RNA interference;
RNA,small interfering gene expression;
liposomes
- From:
Tianjin Medical Journal
2009;37(7):529-531,后插1
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the interference effect of lipofectamine-mediated short hairpin RNA (shRNA) on the expression of PIM-1 gene in prostate cancer cell lines. Methods: Three kinds of recombinant plasmid expression vectors (pP[M]- hRNA-1,-2,-3) were constructed, which can produce shRNA targeting different sequence of PIM-1 mRNA. The recombinant plasmid vectors were transfected into PC-3 cell lines, respectively. RT-PCR and Western blot assay were used to detect the expression of PIM-1 mRNA and protein after transfection. Results: The expression of mRNA and protein of PIM-1 in PC-3 were inhibited significantly at 48h post-transfection. Furthermore, pPLM1- hRNA-2 and pPIM1- hRNA-3 resulted in a stronger silencing effect than that of pPIM- hRNA-1. Conclusion: The recombinant plasmid expression vector which carrying PIM-1 shRNA may remarkably inhibit the expression of PIM-1 mRNA and protein in PC-3 cell lines. The experiment provided the basis for further investigation of the role of PIM-1.
