Comparison of HLA serologicul typing and molecular typing in acute leukemia between pre-and post complete remission
10.3760/cma.j.issn.1009-9921.2008.06.006
- VernacularTitle:急性白血病缓解前后HLA分子血清学分型和分子生物学分型的比较
- Author:
Jia GAN
;
Ti SHEN
- Publication Type:Journal Article
- Keywords:
Leukemia,myelocytic,acute;
Leukemia,lymphocytic,acute;
Histocompatibilitytesting;
Hematopoietic stem cell transplantation
- From:
Journal of Leukemia & Lymphoma
2008;17(6):418-420,423
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the results of HLA serological typing and gene typing in acuteleukemia before and after achieved complete remission.then make sure whether the HLA matching could be performed before remission of acute leukemia.Methods The peripheral blood samples of 20 patients with acute leukemia at onset and in complete remission were performed for the HLA serological typing and gene typing by complement-dependent microcytotoxicity(CDC)assay and PCR-sequence for specific primer. The class Ⅰ HLA antigens reaction was quoted as NIH score.and paired-sample t test Was performed.The class Ⅰand class Ⅱ HLA allele specificity of acute leukemia at onset Was compared with that in complete remission.Results No absence or change of the class Ⅰ and class Ⅱ HLA allele specificity in acute leukemia was observed before treatment and after complete remission.There were significant differences (P＜0.05) for HLA-A. B and Bw6 reaction at onset from complete remission.The expression of HLA-A, B and Bw6 was significant down-regulated.This phenomenon did not occur on Bw4 (P＞0.05).The relationship of down-regulation of class Ⅰ HLA antigens and the amount of blast in peripheral blood Was not observed.Conclusion HIA typing could be performed by molecular technique when patients with acute leukemia were diagnosed.It would shorten the period of HLA-matching,and increase the opportunity of finding an appropriate allergenic hematopoietic stem cell donor.
