Application of sorting peripheral blood cells by flow cytometry in the detection of peripheral Mood cell sorting

VernacularTitle:流式细胞术分选外周血细胞方法的研究
Author: Meijing ZHENG ; Jingjing DUAN ; Liping SU ; Yanfeng WANG ; Wen SU
Publication Type:Journal Article
Keywords: Flow cytometry; T-lymphocytes; Granulocytes
From: Journal of Leukemia & Lymphoma 2010;19(3):159-160,165
CountryChina
Language:Chinese
Abstract: Objective To apply fluo-rescencc-activated cell sorting(FACS) in sorting T lymphocyte (CD_3~+) and granulocyte (CD_(15)~+), which establish the separating method of series of cells from human peripheral blood, so that the scientific research and clinical research could be carried out were specifically. Methods 10 cases of normal peripheral blood were collected and T lymphocyte (CD_3~+) and granulocyte (CD_(15)~+) were stained with florescence conjugated antibodies. The positive cells were sorted by FACS. Results Before sorting the peripheral blood, the proportion of the T lymphocyte(CD_3~+) and granulocyte (CD_(15)~+) in the leukocyte is 48.8 % and 30.8 %; after sorting by FACS, the purity of T lymphocytes (CD_3~+) is up to 98 % and the recovery is about 95 %; the purity of granulocyte (CD_(15)~+) is up to 97 % and the recovery is about 96 %. Conclusion The FACS could Mlow us to quickly sort T lymphocyte (CD_3~+) and granulocyte (CD_(15)~+) with higher recovery and higher purity from the peripheral blood.