Study on the relationship between CRKL activity and multidrug resistance in leukemia cell lines
10.3760/cma.j.issn.1009-9921.2009.07.001
- VernacularTitle:CRKL活性与白血病细胞多药耐药的关系
- Author:
Shaohua SHEN
;
Chunhua QI
;
Longjun GU
;
Xianyong YANG
;
Xin YE
- Publication Type:Journal Article
- Keywords:
Leukemia;
Proto-Oncogenes;
Phosphorylases
- From:
Journal of Leukemia & Lymphoma
2009;18(7):385-387,391
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of CRKL activity in leukemia cells with muhidrug resistance and find new factor related to multidrug resistance. Methods By flow cytometry, CRKL activity was compared in K562, HL-60 cells and its resistance cells. The change of CRKL activity was observed in sensitive cells treated with and withdrawal daunorubicin. Results With the comparison of K562, HL-60 sensitive cells, in K562, HL-60 resistant cell lines, the level of CRKL phosphorylation in K562, HL-60 resistance cells treated with daunombicin 72 hours increased markedly. The level of CRKL phosphorylation was time-dependent with chemotherapy drugs, not change of CRKL activity was found in Jurkat ceils.Conclusion The level of CRKL activity is new factor related to muhidrug resistance in leukemia cells.
