Use of interphase fluorescence in situ hybridization for diction of trisomy 8 in hematologic disorders
10.3760/cma.j.issn.1009-9921.2008.02.006
- VernacularTitle:间期荧光原位杂交检测血液病染色体三体8
- Author:
Shuqin CHENG
;
Chengjian CHEN
;
Weicheng XIE
;
Bixia XIE
;
Zhaohui HUANG
;
Xiaolong CAO
- Publication Type:Journal Article
- Keywords:
Hematologic diseases;
In situ hybridization,fluorescence;
Chromosomes;
Trisomy 8
- From:
Journal of Leukemia & Lymphoma
2008;17(2):98-99,104
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the value of interphase fluorescence in situ hybridization(FISH) in the detection of trisomy 8 in patients with hematologic disorders. Methods Seventy-seven patients were vestigated by directly labeled centrome DNA probes specific for 8 chromosome. The results were compared with that of conventional cytogenetic (CC) analysis. Results The proportion of trisomy 8 of 77 cases of hematologic disorders detected by FISH is higher than by G-banding karyotyping and FISH could offer the result when conventional cytogenetic methods failed to diagnose. Conclusion Interphase FISH is more sensitive in the detection of trisomy 8 than CC, and FISH displays its superiority in the detection of small clone.
