Evaluation of in vitro cytotoxicity and drug release property on pharmaceutical dressing
- VernacularTitle:载药敷料体外细胞毒性及释药性评价
- Author:
Shujie HUANG
;
Jing GUAN
;
Zhihong LI
;
Xizheng ZHANG
;
Jimin WU
- Publication Type:Journal Article
- Keywords:
Proliferation;
Levofloxacin;
Release rate;
Cytotoxicity;
Drug release
- From:
Journal of Medical Biomechanics
2010;25(1):32-35,39
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the cellular toxic and release of pharmaceutical dressing.Method Following the State standard GB/T14233.2-2005.the L929 cellular merphology was observed by inveded microscopy after 72h and proliferation of the cells was examined using mitochondrial function(MTT)assay.Relative growth rate (RGR)was calculated and cytotoxicity grade was evaluated by absorbency(OD)data.With PBS7.4 as dissolution media,and(32±0.5)℃as dissolution temperature,the release rate was determined with UV method with the determination wavelength of 288 nm and the dissolved liquid in 1/6,1/2,1,3,16,24,36 and 48 h.Results The average cell RGR of the pharmaceutical dressing is 91.25%and reaches 1 cytotoxicity grade.L929 cellular morphology is normal.Pharmaceutical dressing release accord to Higuchi equation,and the simulated equation is M_t/M_∞=0.3271t~(0.239).Conclusions Biologic compatibility of the pharmaceutical dressing is good,and the release of levofloxacin from the pharmaceutical dressing is sustained in vitro.
