Apoptosis of leukemia Molt-4 cells induced by resveratrol and effect of WAVE1 gene expression
10.3760/cma.j.issn.1009-9921.2011.07.002
- VernacularTitle:白藜芦醇诱导白血病Molt-4细胞凋亡及对WAVE1基因表达的影响
- Author:
Yongjun LI
;
Junxia YAO
- Publication Type:Journal Article
- Keywords:
Neoplasms,experimental;
Resveratrol;
Molt-4 cells;
Apoptosis;
WAVE1
- From:
Journal of Leukemia & Lymphoma
2011;20(7):389-391,394
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the possible mechanism of apoptosis effect induced by resveratrol in human acute T lymphoblast leukemia Molt-4 cells. Methods MTT method was used to analyze the inhibition rate. The cell cycle and apoptosis percentage of Molt-4 cells treated with resveratrol were detected by flow cytometry. The expression of WAVE1 mRNA was assessed by semi-quantitative RT-PCR. Results After treated with 12.5, 25.0, 50.0, 100.0, 200.0μmol/L resveratrol for 24, 48 and 72 hours, the inhibitory effects were at 29.32 %, 36.11 %, 53.92 %, 62.50 %, and 74.98 %, respectively. Resveratrol was able to inhibit the proliferation of Molt-4 cells in a time-dependent and dose-dependent manner (F =33.614, P <0.05).Compared with control group, after treated with 50.0, 100.0 μmol/L resveratrol for 48 h, the cell number in S phase of Molt-4 cells was 68.6 % and 78.1 %, respectively, and the cell cycle was arrested at S phase (F = 19.453, P < 0.01) after treated with 50.0, 100.0 μmol/L resveratrol for 48 h, the ratio of WAVE1/GAPDH was 0.356±0.03, 0.382±0.05. Compared with control group 0.586±0.06, the ratio of WAVE1/GAPDH was decreased (F =8.950, P <0.01). Conclusion Resveratrol could induce the cells to undergo apoptosis, which was probably related to downregulation the expression of WAVE1.
