Study on the deletion of derivative chromosome 9 in chronic myeloid leukemia patients with extra-signal or dual colour/dual fusion probe, and local-specific inspector 9q34 probe

VernacularTitle:应用LSI9q34探针及ES/DCDF探针检测慢性粒细胞白血病患者der(9)缺失的研究
Author: Linying GAO ; Ping MAO ; Yi YING ; Qinghua DU ; Xiaoyan CHEN
Publication Type:Journal Article
Keywords: ES/DCDF probe; LSI 9q34 probe; Leukemia,myeloid,chronic; Sequence deletion
From: Journal of Leukemia & Lymphoma 2011;20(4):206-208,217
CountryChina
Language:Chinese
Abstract: Objective To study the frequency of the derivative chromosome 9 [der(9)] deletion among patients with chronic myeloid leukemia (CML), and explore the application value of local-specific inspector (LSI) 9q34 probe in detect the der (9) deletion. Methods Cytogenetic analysis was performed by 24 h unstimulated culture, GTG banding and karyotype. Dual colour/dual fusion or extra signal(ES) DNA probe was used to perform interphase-FISH for the detection of bcr-abl fusion gene in 52 patients with CML. LSI 9q34 DNA probe was used to detect der(9) deletion. Results FISH results showed bcr-abl fusion gene existed in all 52 patients with CML. der(9) deletion was detected in 12 patients with ES/DCDF probe, but only in 11 patients with LSI 9q34 probe. Conclusion It's more efficient in detection of der(9) deletion with LSI 9q34 probe. Deletion of der(9) might be a poor prognostic factor for CML patients, and LSI 9q34 probe should be used in all the CML patients with positive bcr-abl fusion gene.