Impact and significance of hypoxic preconditioning in the expression of apoptosis-related protein in remaining liver tissue of rats after hepatic resection
10.3760/cma.j.issn.1673-4203.2014.11.011
- VernacularTitle:低氧预适应对大鼠肝切除后残肝组织凋亡相关蛋白表达的影响及意义
- Author:
Shichun ZHU
;
Xinnong LIU
;
Peijian ZHANG
;
Jian WANG
;
Pengfei LI
;
Xiaofan HAN
- Publication Type:Journal Article
- Keywords:
Hypoxic preconditioning;
Hepatectomy;
Cytochromes;
Caspase 3;
Ischmia-reperfusion injury;
Cytoprotection
- From:
International Journal of Surgery
2014;41(11):754-758,封4
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the impact and significance of hypoxia preconditioning on the expression of cytochrome C and caspase 3 protein in rats after hepatic resection.Methods A hepatectomy model was used to study the ischemia reperfusion injury in hepatic resection.Sprague-Dawley rats were randomly divided into the following three groups:normal control (NC) group,hepatic resection(HR) group,and hypoxia preconditioning (HP) group,there were twenty four rats in each group.HP Group was given an 10％ oxygen-mixed gas for 90 minutes before the operation.At 1,6,12 and 24 hours after the operation,the rats were killed and the following tests were conducted:(1) Liver tissue was sampled to observe the expression of cytochrome C and caspase 3 protein; (2) blood was drawn to conduct a chemical examination; (3) Liver tissue and morphology was observed by transmission electron microscopy.Results The serum levels of ALT and AST in HP group were significantly lower than that of HR group (P＜0.05) at 1,6,12 and 24 hours after the hepatic resection.In each time,liver function of the HP group was significantly better than the HR group; The expression of cytochrome C and caspase 3 protein was decreased significantly in HP group at each measurement point.Hepatic cells in HR group showed typical apoptosis signs under transmission electron microscopy (TEM),but no apoptosis was found in HP group.Conclusion HP has marked inhibition to apoptosis by down-regulating the expression of Cyt C and Caspase-3protein and protection to chondrosomes after a hepatic resection.
