Preparation and cytotoxicity study of multi-wailed carbon nanotubes modified with low-molecular-weight chitosan
10.3760/cma.j.issn.1673-4181.2015.01.003
- VernacularTitle:低分子质量壳聚糖修饰多壁碳纳米管的制备及细胞毒性研究
- Author:
Xia DONG
;
Lanxia LIU
;
Dunwan ZHU
;
Hailing ZHANG
;
Xigang LENG
- Publication Type:Journal Article
- Keywords:
Chitosan;
Multi-walled carbon nanotubes;
Cellular uptake;
Cytotoxicity
- From:
International Journal of Biomedical Engineering
2015;38(1):11-14,后插3
- CountryChina
- Language:Chinese
-
Abstract:
Objective To prepare stable aqueous dispersions of chitosan/multi-walled carbon nanotubes (CS/MWCNTs) composites,and observe the effects of CS/MWCNTs on the growth of human umbilical vein endothelial cells (HUVEC).Methods CS/MWCNTs composites were prepared by electrostatic interactions between negatively charged MWCNTs and positively charged low-molecular-weight CS.The prepared CS/MWCNTs were characterized by transmission electron microscopy and Zetasizer nano-analyser.The cellular uptake of the fluorescently labeled CS/MWCNTs was observed by laser confocal microscopy after incubating with HUVEC for 24 h at different concentrations.In vitro cytotoxicity and cellular reactive oxygen were also detected.Results When the mass ratio of low-molecular-weight CS to MWCNTs was equal or greater than 10∶1,the CS/MWCNTs can be stabilized in solution.Cellular uptake experiments showed that the CS/MWCNTs could enter into the cells and locate mainly in the cytoplasm.Cytotoxicity study showed that the CS/MWCNTs composites was less toxic than MWCNTs alone at high concentration (10 and 20 μg/ml).However,there was no significant differencein the level of cellular reactive oxygen between the two groups (P＜0.05).Conclusions CS/MWCNTs composites showed low cytotoxicity and high stability,which would be a promising carrier for drug delivery.
