In vitro differentiation of islet-like cells from mouse bone-marrow stem cells on a PLGA scaffold
10.3760/cma.j.issn.1673-4181.2014.05.002
- VernacularTitle:体外PLGA三维支架上诱导小鼠骨髓干细胞向胰岛样细胞分化
- Author:
Yimin SONG
;
Chen LIN
;
Xiaobin LI
;
Bing MU
;
Yupei ZHAO
- Publication Type:Journal Article
- Keywords:
Bone-marrow stem cells;
Islet;
Poly (lactic-co-glycolic acid) scaffold
- From:
International Journal of Biomedical Engineering
2014;37(5):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigated the potential influences of poly(lactic-co-glycolicacid)(PLGA)scaffold as a platform on the differentiation of mouse bone-marrow stem cells to islet-like cells.Methods Mouse bonemarrow stem cells were grown and differentiated in culture with or without PLGA scaffold,and cell morphology and functions were compared within these groups.Results The PLGA scaffold showed fine biological compatibility.Differentiated islet-like cells were dithizone (DTZ) positive,insulin and C-peptide double positive,glucagon positive and somatostatin positive in both groups.Under electron microscope there were ultrastructures similar to that of islet β cells in cells of both groups.Cells with PLGA scaffold secreted more insulin under high level glucose stimulation (P＜0.01).Conclusions PLGA scaffold was biologically compatible and improved function of the differentiated islet-like cells.
